To do this, buffer solutions must contain both a weak acid ... This buffer should have a pH of 4.7 ± 0.5 with the ability to stay within one pH unit of this target when strong acid or base is added.

Determining Buffer Components for a Desired pH -A theoretical explanation of how buffers ... titration procedure used to determine the buffering capacity of a solution. Data for 4 different Buffer ...

The stock solution provided is 10 µmol/ml. As p-nitrophenol is a skin irritant, you should wear gloves throughout these assays. 2. Prepare a sub-stock by diluting 100 µl of original (10 µmol/ml) to ...