CRISPR construct to genetically ablate the GABA transporter GAT3 in the mouse visual cortex, with effects on population-level neuronal activity. This work is important, as it sheds light on how GAT3 ...

The rapid identification of respiratory virus outbreaks is needed to enable rational and effective public health interventions. We developed new quantitative approaches for simultaneous ...

Here we use cryogenic electron microscopy to determine the structure of TbAQP2 from Trypanosoma brucei, bound to either the substrate glycerol or to the sleeping sickness drugs, pentamidine or ...

Beads were then resuspended in 100 μL Co-IP wash buffer (+10 mM MgCl 2) and treated with 2 μL Turbo DNase (Thermo Fisher Scientific) and, optionally, 5 μL of a 1:50 dilution of RNase I (Ambion) for 10 ...